AlphaBioCoat

Description

Product Name AlphaBioCoat
Catalog Number AC001
Product Format Liquid
Storage 2-8° C

*Caution: The handling of human derived products has potential to be biologically. All Cell strains tested negative for HIV, HBV, and HCV DNA
in diagnostic tests. Proper precautions must be taken to avoid exposure. Always wear proper protective equipment (Gloves, safety glasses, etc.)
when handling these materials. We recommend following the universal procedures for handling products of human origin as the minimum
precaution against contamination.
GENERAL INFORMATION
Cell culture can be a very difficult tool to work with in the laboratory. Universal BioProducts often receives calls
from anxious customers who are experiencing growth or attachment problems with their cell lines.
Growth and attachment problems with cell lines are caused by:
1. Poor surface treatment
2. Culture media
3. Sera
4. High conductivity of the vessels
Universal BioProducts has spent many years helping customers dealing with these problems. Making your cell
behave is not that hard. It’s all about positive reinforcement. The best product that will do extracellular
matrix (ECM) that provides structural and biochemical support to the surrounding cells. It facilitates cell
growth and attachment.
Our product unique characteristics:
Product Name AlphaBioCoat
Catalog Number AC001
Product Format Liquid
Storage 2-8° C
2 years shelf life
Great for Coating plates, and T-flasks
Work very well when used in 3D tissue models
From gel in a few minutes after neutralizing the product
Purity > 98% (SDS PAGE)

Product uses:
A) Coating a T-25 flask: add 2ml of AlphaBioCoat solution into one T-25 flask and make sure whole
surface is covered with the coating solution.
B) Place Flask in a 37degrees Incubator for Thirty minutes, dispose AlphaBioCoat solution by
aspiration and the flask is ready to be used (no need for overnight incubation when coated with
AlphaBioCoat solution)
C) Gently rinse the cells in T-25 flask with 5ml1XPBS (Code 300) room temperature (RT) twice.
Make sure the plate is at a neutral pH before seeding cells into T-25 flask.
D) Remove Cells from the liquid Nitrogen, place in a water to thaw cells. (Note: Don’t spin the cells
during the subculture process.
E) Suspend the cells with 5ml of Media. Transfer Cells suspension directly into 1X pre-coated T-25
Flask.
F) Place Cells in a 37 Degrees Incubator.
G) Cell Culture Protocol (Proliferating).
H) Culture Media is changed every 2 days

International:

May vary, we will contact you!