Anti – BIN1 Antibody (Monoclonal )

Description

Product Name Anti-BIN1 antibody (monoclonal)
Description Mouse IgG monoclonal antibody for BIN1, bridging integrator 1
(BIN1) detection. Tested with WB, IHC-F, ICC in Human; mouse
rat. No cross reactivity with other proteins.
Host Mouse
Target Species Human, Mouse, Rat
Application WB,HC-P, IHC-F, ICC,
Recommended
Detection
Systems
We recommends Enhanced Chemiluminescent Kit with anti-Mouse
IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse
IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and
ICC.
*Blocking peptide can be purchased. Contact us for more
information
Immunogen Recombinant polypeptide containing amino acids 189-398 of
human Bin1.
Specificity No cross reactivity with other proteins
Pack Size 100μg/vial
Catalog Number Ab1005

Background:
BIN1(AMPH2) is a novel human gene product with features of a tumor suppressor
protein. BIN1 gene to chromosome 2q14. Loss of BIN1 expression appears to be a
frequent aberration in human hepatocellular carcinomas . mutations in BIN1 cause
centronuclear myopathy by interfering with remodeling of T tubules and/or endocytic
membranes, and that the functional interaction between BIN1 and DNM2 is necessary
for normal muscle function and positioning of nuclei.
Product Properties:
Clonality Monoclonal
Clone Number 99D
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg
NaN3 as preservative.
*carrier free antibody available upon request.
Reconstitution Add 1ml of 1XPBS buffer (catalog # 1XPBS-001) will yield a
concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It
can also be aliquoted and stored frozen at -20˚C for a longer time.
Avoid repeated freezing and thawing.
Purification Ascites
Application Details:
Application Concentration* Tested
Species
Predicted
Species
Antigen
Retrieval
Western blot 0.25μg/ml Human,
Mouse,
Rat
–
Immunohistochemistry(Frozen
Section)
0.5μg/ml Human,
Mouse,
Rat
–
Immunocytochemistry 1μg/ml Human,
Mouse,
Rat

Protein Target:
Gene Name BIN1
Protein Name Myc box-dependent-interacting protein 1
Gene Full Name bridging integrator 1
Protein Function May be involved in regulation of synaptic vesicle endocytosis.
May act as a tumor suppressor and inhibits malignant cell
transformation.
Tissue Specificity Ubiquitous. Highest expression in the brain and muscle. Isoform
IIA is expressed only in the brain where it is concentrated in
axon initial segments and nodes of Ranvier. Isoform BIN1 is
widely expressed with highest expression in skeletal muscle.
Sequence
Similarities
Contains 1 BAR domain.
Subcellular
Localization
Isoform BIN1: Nucleus.
Uniprot ID Human(O00499), Mouse(O08539), Rat(O08839)
Alternative Names BIN1

International:

May vary, we will contact you!