Anti-Calcineurin Alpha Antibody(Monoclonal, CN-A1)

Description

Product Name Anti-Calcineurin Alpha Antibody (Monoclonal, CN-A1)
Description Mouse IgG monoclonal antibody for Calcineurin alpha, protein
phosphatase 3, regulatory subunit B, alpha (PPP3R1) detection.
Tested with WB, IHC-P in Human;bovine;rat. No cross reactivity
with other proteins.
Host Mouse
Target Species Bovine, Human, Mouse, Rat
Application IHC-P, WB
Recommended
Detection
Systems
We recommends Enhanced Chemiluminescent Kit with anti-Mouse
IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse
IgG Super Vision Assay Kit (SV0001-1) for IHC(F). *Blocking
peptide can be purchased. Contact us for more information
Immunogen Bovine brain calcineurin.
Specificity No cross reactivity with other proteins
Pack Size 100μg/vial
Catalog
Number
Ab1008
Background:
Calcineurin, the Ca(2+)/calmodulin-regulated protein phosphatase, first detected in
skeletal muscle and brain, has been found in all cells from yeast to mammals.
Calcineurin A alpha(PPP3CA),is located on human chromosomes 4, Chromosomal
mapping of the human genes for the calmodulin-dependent protein
phosphatase(calcineurin) catalytic subunit. Calcineurin regulates bone formation by the
osteoblast.

Product Properties:
Clonality Monoclonal
Clone Number CN-A1
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg
NaN3 as preservative. *carrier free antibody available upon request.
Reconstitution Add 1ml of 1XPBS buffer (catalog # 1XPBS-001) will yield a
concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It
can also be aliquoted and stored frozen at -20˚C for a longer time.
Avoid repeated freezing and thawing.
Purification Ascites
Application Details:
Application Concentration
*
Tested
Species
Predicted
Species
Antigen
Retrieval
Western blot 0.25-0.5μg/ml Human,
bovine,
mouse,
rat
–
Immunohistochemistry(Paraffin
-embedded Section)
l 0.5-1μg/ml Human,
bovine,
mouse,
rat
– By Heat

Protein Target:
Gene Name PPP3R1
Protein Name Calcineurin subunit B type 1
Gene Full Name protein phosphatase 3, regulatory subunit B, alpha
Protein Function Regulatory subunit of calcineurin, a calcium-dependent,
calmodulin stimulated protein phosphatase. Confers calcium
sensitivity.
Sequence
Similarities
Belongs to the calcineurin regulatory subunit family.
Subcellular
Localization
Cytoplasm, cytosol . Cell membrane . Cell membrane,
sarcolemma. Note: Translocates from the cytosol to the
sarcolemma in a CIB1- dependent manner during
cardiomyocytes hypertrophy.
Uniprot ID Human(P63098), Mouse(Q63810), Rat(P63100)
Alternative Names Calcineurin subunit B type 1|Calcineurin
alpha|PPP3R1|CalcineurinsubunitBtype1|Calcineurinalpha

International:

May vary, we will contact you!