Neonatal Rat Cardiac Fibroblasts

Description

Product Name: Neonatal Rat Cardiac Fibroblasts
Catalog No: R1015
Product Format: Frozen or Proliferating
Cell Number: >90% confluent in T-25 flask/Frozen vial
Storage: 37°C or liquid Nitrogen
General Information: Neonatal Rat Cardiac Fibroblasts cells were isolated from
normal neonatal SD rats heart tissue. Passage 1 Neonatal Rat Cardiac Fibroblasts
cell can be shipped proliferated in a T-25 flask at >90 confluence, or frozen vial (> 5
x 105 cells). DMEM contains 5% Fetal calf serum (Full medium) is recommended for
cell culture and these cells have an average minimum population doubling levels >8
when cultured following the detailed protocol. Cells are tested negative for common
experimental animal pathogens, and mycoplasma in vitro. When you receive the
cells, leave the flask in 37°C CO2 incubator for 1 hour first, and then replace the
transport medium with fresh Full medium. Let the cells grow for 24hours before
subculture.
Subculturing Proliferated Cells:
1. Rinse the cells in T25 flask with 5ml DPBS (Room Temperature, RT) twice.
2. Add 2ml of Trypsin/EDTA (RT) into T25flask (make sure the whole surface of
the T25 flask is covered with Trypsin/EDTA),and gently dispose the
Trypsin/EDTA solution within 10 seconds with aspiration.
3. Leave the T25 flask with the cells at RT for 1 minute (the cells will normally
come off the surface within 1-2 minute). Suspend the cells with 40ml of fresh
Full medium and the cell suspension is transferred directly into 4 x T25 flasks
(10ml each, and the cells are subcultured at 1:4ratio).
4. Culture medium (full medium) is changed every 2-3 days. The cells normally
become confluent within 7 days (when split with a ratio of 1:4).

Product Information:
Tissue Origin: SD rats heart tissue.
Passage: 1
Storage Conditions: Liquid Nitrogen− Vapor Phase Only
Special Handling: Minimize exposure to non−liquid nitrogen temperatures, especially room
Temperature transfers.
Date of Expiration: Guaranteed for up to 6 months from date of shipment when stored in
Liquid Nitrogen.
Required Testing and Results
Test Specification Results
Cell Count > 1x105viable cells/vial ~1.90×105 Cells/Vial
Cell Viability >90% 98%
Endotoxin <3 EU/mL <1 EU/mL
USP Sterility

Negative Negative
Mycoplasma Negative Negative
Appearance, Container
Integrity
Conforms Conforms
Appearance, Suspension
Appearance
Conforms Conforms
Product Handling:
Caution: The user should treat all cells as potential pathogens. Wear protective clothing and
eye wear. Practice appropriate disposal techniques for potentially pathogenic or
biohazardous materials.
For Research Use Only. Not for use in diagnostic procedures. If you have any further
questions about this Certificate of Analysis, please contact us